Antioxidant and Anticholinesterase activities of Stachys pumila
Biological activities of Stachys pumila
DOI:
https://doi.org/10.1071/ejmbs.v4i2.25Keywords:
Antioxidant, anticholinesterase, Stachys, Medicinal PlantsAbstract
Numerous plant species have been utilized in traditional medicine since antiquity. Plants are essential natural resources for those possessing these attributes. The total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index of Stachys pumila were assessed in our study. The inhibitory activities of acetylcholinesterase and butyrylcholinesterase in the plant were assessed. The aerial components of the plant were extracted using ethanol in a Soxhlet system. The TAS, TOS, and OSI values were ascertained with Rel Assay kits. Anticholinesterase activity were assessed using the Elman technique. The experiments determined the TAS value of the plant's ethanol extract to be 5.447±0.024 mmol/L, the TOS value to be 6.974±0.043 μmol/L, and the OSI value to be 0.128±0.001. The anti-AChE value was measured at 38.28±1.95 μg/mL, whereas the anti-BChE value was measured at 63.12±1.54 μg/mL. It was established that the plant's biological activities were within normal ranges and that it possesses potential for pharmaceutical applications.
References
Akgul, H., Korkmaz, N., Dayangaç, A., Sevindik, M. (2020). Antioxidant potential of endemic Salvia absconditiflora. Turkish Journal of Agriculture-Food Science and Technology, 8(10), 2222-2224.
Akgül, H., Sevindik, M., Akata, I., Altuntaş, D., Bal, C., Doğan, M. (2016). Macrolepiota procera (Scop.) Singer. mantarının ağır metal içeriklerinin ve oksidatif stres durumunun belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20(3), 504-508.
Bal, C., Eraslan, E. C., Sevindik, M. (2023). Antioxidant, antimicrobial activities, total phenolic and element contents of wild edible mushroom Bovista nigrescens. Prospects in Pharmaceutical Sciences, 21(2), 37-41.
Çömlekçioğlu, N., Korkmaz, N., Yüzbaşıoğlu, M. A., Uysal, İ., Sevindik, M. (2024). Mistletoe (Loranthus europaeus Jacq.): antioxidant, antimicrobial and anticholinesterase activities. Prospects in Pharmaceutical Sciences, 22(3), 164-169.
Daştan, S.D., Durukan, H., Demirbaş, A., Dönmez, E. (2019). Bioactivity and Therapeutic Properties of Evelik (Rumex crispus), A Naturally Growing and Edible Plant in Sivas Province. Turkish Journal of Agriculture-Food Science and Technology, 7(sp2): 67-71.
Davis, P.H. (1982). Flora of Turkey and the East Aegean Islands. Vol. 7. Edinburgh University Press, Edinburgh, UK p. 227
Dursun, A., Çalışkan, O., Güler, Z., Bayazit, S., Türkmen, D., Gündüz, K. (2021). Effect of harvest maturity on volatile compounds profiling and eating quality of hawthorn (Crataegus azarolus L.) fruit. Scientia Horticulturae, 288, 110398.
El-Chaghaby, G. A., Mohammed, F. S., Rashad, S., Uysal, I., Koçer, O., Lekesiz, Ö., Dogan, M., Şabik, A.E., Sevindik, M. (2024). Genus Hypericum: General Properties, Chemical Contents and Biological Activities. Egyptian Journal of Botany, 64(1), 1-26.
Ellman, G. L., Courtney, K. D., Andres Jr, V., Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95.
Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical biochemistry, 37(4): 277-285.
Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry, 38(12): 1103-1111.
Ferhat, M., Erol, E., Beladjila, K. A., Çetintaş, Y., Duru, M. E., Öztürk, M., Kabouche, A., Kabouche, Z. (2017). Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica. Pharmaceutical biology, 55(1), 324-329.
Güler, Z., Dursun, A., Türkmen, D. (2022). Comparison of pistachio (Pistacia vera L.) kernel volatile compounds of “Siirt”,“Uzun” and “Kirmizi” grown in Turkey: Effect of traditional and industrial in‐shell roasting. Journal of Food Process Engineering, 45(2), e13957.
Gürgen, A., Sevindik, M., Krupodorova, T., Uysal, I., Unal, O. (2024). Biological activities of Hypericum spectabile extract optimized using artificial neural network combined with genetic algorithm application. BMC biotechnology, 24(1), 83.
Islek, C., Saridogan, B. G. O., Sevindik, M., Akata, I. (2021). Biological activities and heavy metal contents of some Pholiota species. Fresenius Environmental Bulletin, 30(6), 6109-6114.
Karaman, S., Ilcim, A., Comlekcioglu, N. (2007). Composition of the essential oils of Salvia aramiensis Rech. fil. and Salvia cyanescens Boiss. & Bal. Pakistan Journal of Botany, 39(1), 169.
Karcioglu, L., Tanis, H., Comlekcioglu, N., Diraz, E., Kirecci, E., Aygan, A. (2011). Antimicrobial activity of Salvia trichoclada in southern Turkey. International Journal of Agriculture and Biology, 13(1), 134-136
Kepekçi, R.A., Polat, S., Çoşkun, G., Çelik, A., Bozkurt, A. S., Yumrutaş, Ö., & Pehlivan, M. (2017). Preliminary characterization of phenolic acid composition and hepatoprotective effect of Stachys pumila. Journal of Food Biochemistry, 41(2), e12286.
Korkmaz, N., Mohammed, F. S., Uysal, İ., Sevindik, M. (2023). Antioxidant, antimicrobial and anticholinesterase activity of Dittrichia graveolens. Prospects in Pharmaceutical Sciences, 21(4), 48-53.
Manzione, M. G., Herrera‐Bravo, J., Sharifi‐Rad, J., Kregiel, D., Sevindik, M., Sevindik, E., Selamoplu, Z., Zam, W., Vitalini, Z., Hano, C., Kukula-Koch, W., Koch, W., Pezzani, R. (2022). Desmodium adscendens (Sw.) DC.: A magnificent plant with biological and pharmacological properties. Food Frontiers, 3(4), 677-688.
Mohammed, F. S., Korkmaz, N., Doğan, M., Şabik, A. E., Sevindik, M. (2021). Some medicinal properties of Glycyrrhiza glabra (Licorice). Journal of Faculty of Pharmacy of Ankara University, 45(3), 524-534.
Mohammed, F. S., Sevindik, M., Uysal, I., Sevindik, E., Akgül, H. (2022). A natural material for suppressing the effects of oxidative stress: biological activities of Alcea kurdica. Biology Bulletin, 49(Suppl 2), S59-S66.
Mohammed, F. S., Uysal, I., Sevindik, M. (2023). A review on antiviral pants effective against different virus types. Prospects in Pharmaceutical Sciences, 21(2), 1-21.
Özcandır, A., Mohammed, F. S., Sevindik, M., Aykurt, C., Selamoglu, Z., Akgül, H. (2024). Phenolic composition, total antioxidant, antiradical and antimicrobial potential of endemic Glaucium Alakirensis. Sigma Journal of Engineering and Natural Sciences, 42(1), 42-48.
Sabik, A. E., Sevindik, M., Mohammed, F. S., Dogan, M. (2024). A New Natural Source against A549 Lung Cancer Cells Anthemis cotula and its Biological Activities and Phenolic Contents. Pharmaceutical Chemistry Journal, 58(7), 1134-1140
Saraç, H., Demirbaş, A., Daştan, S.D., Ataş, M., Çevik, Ö., Eruygur, N. (2019). Evaluation of Nutrients and Biological Activities of Kenger (Gundellia tournefortii L.) Seeds Cultivated in Sivas Province. Turkish Journal of Agriculture-Food Science and Technology, 7(sp2), 52-58.
Saridogan, B. G. O., Islek, C., Baba, H., Akata, I., Sevindik, M. (2021). Antioxidant antimicrobial oxidant and elements contents of Xylaria polymorpha and X. hypoxylon (Xylariaceae). Fresenius Environmental Bulletin, 30(5), 5400-5404.
Seğmenoğlu, M. S., Koçer, O., Sevindik, M., Korkmaz, N., Yüzbaşıoğlu, M. A., Uysal, İ. Antioxidant, Antimicrobial, and Antialzheimer Activities of Tagetes patula (Asteraceae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1)), 205-212.
Sevindik, M. (2019). The novel biological tests on various extracts of Cerioporus varius. Fresenius Environmental Bulletin, 28(5), 3713-3717.
Sevindik, M. (2021). Anticancer, antimicrobial, antioxidant and DNA protective potential of mushroom Leucopaxillus gentianeus (Quél.) Kotl. Indian Journal of Experimental Biology (IJEB), 59(05), 310-315.
Sevindik, M., Akata, I. (2019). Antioxidant, oxidant potentials and element content of edible wild mushroom Helvella leucopus. Indian Journal of Natural Products and Resources, 10(4), 266-271.
Sevindik, M., Mohammed, F. S., Uysal, I. (2023). Autism: plants with neuro-psychopharmacotherapeutic potential. Prospects in Pharmaceutical Sciences, 21(3), 38-48.
Świątek, Ł., Sieniawska, E., Sinan, K. I., Maciejewska-Turska, M., Boguszewska, A., Polz-Dacewicz, M., Senkardes, I., Guler, G.O., Sadeer, N.B., Mahomoodally, M.F., Zengin, G. (2021). LC-ESI-QTOF-MS/MS analysis, cytotoxic, antiviral, antioxidant, and enzyme inhibitory properties of four extracts of Geranium pyrenaicum Burm. f.: A good gift from the natural treasure. International journal of molecular sciences, 22(14), 7621.
Tepe, B., Degerli, S., Arslan, S., Malatyali, E., Sarikurkcu, C. (2011). Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoterapia, 82(2), 237-246.
Tomou, E. M., Barda, C., Skaltsa, H. (2020). Genus Stachys: A review of traditional uses, phytochemistry and bioactivity. Medicines, 7(10), 63.
Tundis, R., Peruzzi, L., Menichini, F. (2014). Phytochemical and biological studies of Stachys species in relation to chemotaxonomy: A review. Phytochemistry, 102, 7-39.
Turkmen, D., Dursun, A., Caliskan, O., Koksal Kavrak, M., Guler, Z. (2023). Volatile compounds, phenolic content, and antioxidant capacity in Sultan hawthorn (Crataegus azarolus L.) leaves. Journal of Agricultural Science and Technology, 25(5), 1089-1099.
Uysal, I., Mohammed, F. S., Sevindik, M., Şabik, A. E., Sevindik, E., Dogan, M. (2023). Genus Thymbra: a review on their usage areas, phytochemical contents and biological activities. Egyptian Journal of Nutrition, 38(3), 1-12.
