Isolation and identification of Staphylococcus aureus and studying effect of physical and chemical mutagenesis on Hyaluronidase production from Iraqi patients

Mays T. Abdallah, Sahar Medhat Hussein, Aya M. Al-Rahim

Abstract


Isolation and identification of S.aureus bacteria from Iraqi patients using microbiological and molecular identification by 16s rRNA system using PCR amplification.  Secondary metabolite production from wild strains is very low for economical purpose therefor certain strain improvement strategies are required to achieve hundred times greater yield of metabolites. The present study was conducted for enhanced production of hyaluronidase from Staphylococcus aureus S9 by mutagenesis using ultraviolet (UV) radiation, and Mitomycin C (MitC) as a mutagens. Results showed that a hyaluronidase over producer mutant symbol S.aureus S9-63 was obtained after mutagenesis with UV irradiation with higher enzyme specific activity (209.0 U/mg protein) in comparison to the wild type (118.02 U/mg protein). On the other hand, another overproducer mutant symbol S.aureus S9- 121) was obtained after chemical mutagenesis with MitC characterized with high hyaluronidase production. the enzyme specific activity in its crude filtrate was 188.4 U/mg protein in comparison with wild type. It could be concluded that physical mutagenesis using UV irradiation was more efficient than the chemical mutagenesis by MitC to enhance the ability of S.aureus A9 in hyaluronidase production.

 


Keywords


Staphylococcus aureus; Mutant; UV; Mitomycin C

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References


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