In Vitro Investigation of the Natural Antioxidant and Potential Neuroprotective Properties of Sideritis perfoliata
DOI:
https://doi.org/10.1071/ejmbs.v5i2.49Keywords:
Plant bioactives, redox balance, free radical scavenging, enzyme inhibition, neurodegenerative processes, ethanol extractAbstract
In this study, the antioxidant and anticholinesterase biological activities of ethanol extracts of Sideritis perfoliata were evaluated using a comprehensive approach. The total antioxidant capacity (TAS), total oxidant level (TOS), and oxidative stress index (OSI) of the extracts obtained from the above-ground parts of the plant using the Soxhlet method were determined using commercial kits. Furthermore, free radical scavenging activity was assessed using the DPPH method, and reducing power using the FRAP method. Anticholinesterase potential was investigated based on the Ellman method using acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, with galantamine used as a positive control. According to the results obtained, the TAS value of S. perfoliata extract was determined as 8.736 ± 0.180 mmol/L, the TOS value as 15.466 ± 0.180 µmol/L, and the OSI value as 0.177 ± 0.002. DPPH and FRAP analyses yielded values of 179.083 ± 3.075 mg TE/g and 227.557 ± 2.604 mg TE/g, respectively. In anticholinesterase tests, the inhibitory effects of the extract on AChE and BChE enzymes were determined as 60.363 ± 1.050 µg/mL and 80.537 ± 1.519 µg/mL, respectively. The results indicate that S. perfoliata possesses a significant antioxidant capacity and exhibits a moderate but consistent inhibitory effect on cholinesterase enzymes. These findings suggest that the species can be evaluated in pharmacological and nutraceutical applications as a natural antioxidant and potential neuroprotective resource.
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