A Successful Encapsulation Technique for The Production of Synthetic Seeds for Lilium candidum L. Grown Naturally in Turkey

Hilal Büşra Tokgöz, Ergun Kaya, Filiz Altan

Abstract


Lilium candidum L. (white lily), which spreads naturally in Turkey, faces the risk of extinction as a result of the destruction of habitats where it spreads and the continuous removal of bulbs for export purposes. For this reason, it is extremely significant to conduct biotechnological studies in L. candidum, which is very important as a medicinal and ornamental plant species. In vitro techniques can provide the opportunity to preserve plant genetic resources in different forms and different genetic features (synthetic seed). With the help of this method, rapid reproduction of endangered plant species, transfer to in vitro or ex-situ conservation environments is possible. In this study, it is aimed to establish a suitable protocol for the production of synthetic seeds in L. candidum. Accordingly, regeneration was achieved by using the stem bulbils of the plant. An effective encapsulation technique has been created for the production of synthetic seeds in L. candidum with calli, obtained by regeneration. The calculated callus development and embryogenesis rates of encapsulated calli were higher than single ones, 92.5%-88.6%, and 90.4%-77.1% respectively. Our protocol turned out to be successful for synthetic seed production as well as large-scale in vitro clonal propagation of L. candidum.


Keywords


Ca-alginate, commercial production, white lily, artificial seed

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References


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