Hepatic and Renal Biochemical Profile of Male Wistar Rats Following Ingestion of Lacatomtom Drink
Hepatic and Renal Effects of Lacatomtom Drink in Male Rats
Keywords:
Psychoactive drink, liver, alanine transaminase, alkaline phosphatase, kidney, toxicityAbstract
There is rising concern about the physical health risks of unconventional psychoactive drugs. A notable substance in this regard, "Lacatomtom" (LTT), has gained popularity among Nigerian youths. This study evaluated hepatic and renal biochemical profiles of male Wistar rats following ingestion of LTT drink. 25 wistar rats were randomly placed into 5 groups of 5 rats. Group A (control): 0.5ml of distilled water, group B (Vehicle) 0.5ml Lacasera, and groups C, D, and E: 125, 500, and 1000mg/kg LTT drink orally for 30 days. Plasma sera were used to analyse biochemical parameters like alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), total protein, albumin, bilirubin, urea, creatinine, and blood electrolytes after treatments. Histopathological examination was carried out on liver and kidney sections. ALT levels were significantly higher in test group E (1000 mg/kg) than Normal control. LTT drink significantly (P>0.05) decreased ALP, total protein, and albumin levels in experimental group C compared to the control, but not in test groups D (500mg/kg) and E (1000mg/kg). Total and conjugated bilirubin, creatinine, urea, potassium (K+), sodium (Na+), and bicarbonate (HCO3-) levels in the blood did not differ significantly between the normal control and Lacatomtom-treated groups (C, D, and E). However, groups C and D had significantly lower chloride serum levels than the usual control. In conclusion, Lacatomtom may alter the homeostatic balance of the liver in a dose dependent manner. It is therefore recommended that public awareness campaigns be launched to educate adolescents on the potential health risk its consumption poses.
References
Abura-Meerdink, G. A., Albright, D. L. (2023). Effectiveness of Adolescent School-Based Digital Mental Health Interventions: A Systematic Review. Research on Social Work Practice, https://doi.org/10.1177/10497315231201140
Blass, K. G., Thibert, R. J., Lam, L. K. (1974). A study of the mechanism of the Jaffe reaction. Clin. Chem. Lab. Med., 1974, 336-343.
Dumbili, E. W., Ezekwe, E., Odeigah, O. W. (2020). From “Codeine Diet” to “Gutter Water”: polydrug use among Nigerian young adults. Drugs and Alcohol Today, 20(2), 95-107.
Doumas, B. T., Watson, W. A., Biggs, H. G. (1971). Albumin standards and the measurement of serum albumin with bromcresol green. Clinica Chimica Acta, 31(1), 87–96.
Emmanuel, F. T., Akor, S. E., Momoh, S., Owemidu, I. O., Akor, S. E. (2022). Investigation of the Hepatotoxicity of Lacatomtom Drink in Albino Rat. Nigerian Journal of Biochemistry and Molecular Biology, 37(1), 26–31.
Erhun, W. O., Babalola, O. O., Erhun, M. O. (2001). Drug regulation and control in Nigeria: The challenge of counterfeit drugs. J. Health Popul. Dev. Ctries., 4(2), 23–34.
Fawcett, J., Scott, J. (1960). A rapid and precise method for the determination of urea. J. Clin. Pathol., 13(2), 156–159.
Ijediogor, G., Ezea, S., Awodipe, T., Diamond, M., Ekemezie, H. (2018). Drug addiction among the youth: A ticking time bomb. Guardian Saturday Magazine.
Jendrassik, L., Grof, P. (1938). Estimation of total serum bilirubin level by spectrophotometrically in serum and plasma. Biochem. Zeitschrift., 297, 81–89.
Khan, I. S., Dar, K. B., Ganie, S. A., Ali, M. N. (2022). Toxicological impact of sodium benzoate on inflammatory cytokines, oxidative stress and biochemical markers in male Wistar rats. Drug Chem. Toxicol., 45(3), 1345–1354.
Khey, D. N., Stogner, J., Miller, B. L. (2013). Emerging trends in drug use and distribution. Springer
Lawal, A., Suleiman, B. A., Olorukooba, A. A., Hamza, K. L., Usman, B., Abdulkadir, A., Abba, S. K., Yahaya, S., Nass, N. S., Amadu, L. (2024). Prevalence and Factors Associated With Substance Abuse Among Adolescents in Public and Private Secondary Schools in Katsina State, Nigeria.
Lillie, R. D. (1969). Exploration of dye chemistry in Taenzer Unna orcein type elastin staining. Histochemie, 19(1), 1–12.
Manal Said, T., Nawal, A.B. (2012). Adverse effects of monosodium glutamate on liver and kidney functions in adult rats and potential protective effect of vitamins C and E. Food and Nutrition Sciences, 2012.
Mohamad, M., Mohammad, M., Mat Ali, N. A., Awang, Z. (2018). The impact of life satisfaction on substance abuse: delinquency as a mediator. Int. J. Adolesc. Youth, 23(1), 25–35.
Obinna, V. C., Kagbo, H. D., Agu, G. O. (2018). Hepatic and Renal Biochemical profile of albino rats exposed to chloroform and methanol leaf extracts of Portulaca oleracea Linn. European J. Med. Plants, 25(3), 1–14.
Okorie, N., Obeagu, E. I., Adeniran, O. C., Onyema, A. S., Agwu, U. (2022). Codeine Substitute Challenges Drug and Substance Abuse Controls in Nigeria: Histopathology Evaluations of Norvegicus rattus on Lacatomtom. J. Complement. Med. Res., 19(1), 8–22.
Reitman, S., Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Path., 28(1), 56–63.
Roy, A. V. (1970). Rapid method for determining alkaline phosphatase activity in serum with thymolphthalein monophosphate. Clin. Chem., 16(5), 431–436.
Tietz, N. W. (1995). Clinical guide to laboratory tests. In Clinical guide to laboratory tests (pp. 1096-1096).
Van Eygen, I., Vannet, B. Vande, Wehrbein, H. (2005). Influence of a soft drink with low pH on enamel surfaces: an in vitro study. Am. J. Orthod. Dentofacial. Orthop., 128(3), 372–377.
Wheeler, L. A. (1998). Clinical laboratory instrumentation. Medical Instrumentation: Application and Design, 3, 498-577.
